Composite
Part:BBa_K092600
Designed by: EPF-Lausanne Team 2008 Group: iGEM08_EPF-Lausanne (2008-10-19)
TetR cds with RBS and Terminator + pTetR, RFP with RBS
Composite part : combination of the part BBa_K092000 and the BBa_K092300 : The TetR sequence (with a ribosome binding site and terminator) is bound to the part containing the promoter of TetR, a ribosome binding site and the red fluorescent protein (RFP). Thus, the production of RFP directly depends on the production of TetR. The transcription of RFP is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1483
Illegal AgeI site found at 1595 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |